Detection of Serum IgG Specific for Brachyspira pilosicoli and “Brachyspira canis” in Dogs

Detection of Serum IgG Specific for Brachyspira pilosicoli and “Brachyspira canis” in Dogs

Blog Article

Brachyspira pilosicoli (B.pilosicoli) is a pathogen Agitator in pigs, poultry, and humans causing colitis, diarrhea, and poor growth rates.Its role as a canine pathogen is controversial, and the seroprevalence of specific IgG antibodies against B.pilosicoli in dogs is unknown.A further, not yet officially recognized Brachyspira species in dogs is “Brachyspira canis” (“B.

canis“), which is proposed to be apathogenic.This study evaluates enzyme-linked immunosorbent assays (ELISAs) measuring serum IgG antibodies specific for B.pilosicoli or “B.canis” and investigates levels of specific IgG antibodies against B.pilosicoli and “B.

canis” in a cohort of clinical patients presented at an animal referral clinic.These Card Holders ELISAs use detergent-extracted antigens from B.pilosicoli and “B.canis”.To increase analytic specificity, we precipitated the antigens with trichloroacetic acid (TCA) to isolate and concentrate the respective protein fraction.

Our results indicate that a large number of serum IgG antibodies bind to shared epitopes of detergent-extracted antigens of the two spirochaetes.Our data also suggest that dogs might not only carry B.pilosicoli but also have “B.canis”-specific serum IgG antibodies.